Part:BBa_K4789004

1.miR-22-sponge-pepper We designed this part with pepper fluorescence to monitor the expression of miR-22 in cells. The “Pepper” plasmid containing the part sequence of LncRNA MALAT1 in order to reflect miRNA expression in vivo. lncRNAs can interact with miRNAs as “sponges”. And the fluorescence reflect the miRNA expression in the cervical cancer in turn. We tested the sensitivity and specificity of the vectors in Hela cells. In the future, the miRNA-LncRNA MALAT1 complex could be used to screen and detect the cervical cancer. The patients could benefit from our work. The model of the plasmid was listed below (Fig 1).

                   
                        Fig 1. The diagram of miR-22- sponge-pepper