Plasmid

Part:BBa_K4606010:Design

Designed by: Litong, Wu and Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)
Revision as of 02:16, 23 September 2023 by Alex2006 (Talk | contribs) (→‎Source)

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Vector-ZsGreen-miR-3074-5p-AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin. ZsGreen protein is spontaneously expressed and emits green fluorescence after successful transfection, so we can observe the transfection efficiency of the vector.


Source

Plasmids were obtained from Escherichia coli.

References