Part:BBa_K4586026
Loading system (CD63-L7Ae)
This composite part coding for our loading system that is composed of two components: L7Ae, which is a RNA binding protein that have an affinity to C\D box or kink turn coupled present within the 3` end of our cargo.
This RNA binding protein is conjugated to the C terminal end of CD63, which is highly expressed trans membrane protein on exosomes surface.
Usage
This part is implemented in our design to mediate the loading of our therapeutic agent into the engineered exosomes thus by labeling our cargo with a C\D box in the 3` prime end in order to bind to the L7Ae and load the cargo in form of RNA selectively into the exosomes that targets the autoreactive B-cells as shown in figure 1. and figure 2.
Figure 1: This figure illustrates the mechanism of loading our therapeutic agent in the form of mRNA selectively and efficiently into our engineered exosomes secreted form the MSCs,this loading is done through labeling the gene of interest with C\D box a hairpin structure IN THE 3` end this box have high affinity to the RNA binding protein L7Ae that is expressed on the internal surface of the engineered exosomes membrane conjugated to his tagged CD63 protein that is a natural highly expressed transmembrane protein within the exosomes.
Figure 2: This figure shows the design of the biological circuit expressing our loading system on the exosomes membrane ,this system consists of two main component ,First the RNA binding protein L7Ae conjugated to the second component, which is CD3 a transmembrane protein that is naturally expressed on the exosomes membrane.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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