Part:BBa_K4586006
Guide RNA
Part Description
Our guide RNA part is a specific RNA fragment that navigates the Cas system to the RNA or DNA target of interest according to its family and serves to make some changes, such as deletion, insertion, or alteration, to the target RNA or DNA by building complexes with them.
Usage
Our guide RNA is designed to navigate the Cas nuclease toward its specific target gene of interest, to knock-out the BAFF-R gene of auto-reactive B-Cell which is the gene responsible for the survival and maintenance of B-Cell that lead to apoptosis, and limit its pathogenic effect in RA as shown in figure 1.
Figure 1: This figure illustrates the activity of the Cas12k protein into the target autoreactive B-cells through knocking down the B-cell activating factor receptor (BAFF-R) gene that is responsible for B-cells survival and proliferation leading to their apoptosis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |