Part:BBa_K4586001

Mouse notch core

Part Description

This part codes for an enhanced Mouse notch core, which is a notch-derived transmembrane regulatory protein that normally regulates cell fate decisions during a variety of developmental processes. It was first identified in beaded winged flies. The activity of the synthetic notch is determined by the sequential cleavage of its domain carried out by protease enzymes after cell interaction with another cell.

Usage

Mouse notch is implicated in our design as the transmembrane domain of our Syn notch receptor as it transmits the signal that is started by cell-to-cell contact between the external domain and the autoreactive B cells through it’s mechanosensitive quality based on proteolytic cleavage of its domains to release our intra-cellular transcription module (ZF21.16 VP64) that activates the ZF21.16 minCMV promoter controlling the expression of our cargo as shown in figure 1.

Figure 1. shows how the mouse notch core protein within the syn notch receptor is expressed as a transmembrane domain to transmit the signal initiated by interaction between the autoreactive B-cell receptor and our engineered MSC

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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