Coding

Part:BBa_K190027:Design

Designed by: Wilfred Poppinga   Group: iGEM09_Groningen   (2009-08-26)
Revision as of 20:48, 16 October 2009 by Nienke (Talk | contribs) (→‎Source)

MBP-ArsR fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
    Illegal BglII site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI.rc site found at 1102


Design Notes

Adding tobacco etch virus (TEV) cleavage site and linker site to facilitate cloning. Adding a SacI restriction site to facilitate non-expensive cloning.


Source

ArsR was obtained from the E. coli K-12 DH10B genome. MBP was obtained from vector pRE-MBP (kindly provided by Guus Erkens, Membrane Enzymology group, University of Groningen).

References