Composite

Part:BBa_K283040

Designed by: Shi Lei, Nelson Chu, W Ling   Group: iGEM09_HKU-HKBU   (2009-10-16)
Revision as of 20:13, 16 October 2009 by Registry (Talk | contribs)

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invasin (1-625aa)_RFP-terminators

This part generates the chez protein which is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. A 6-histidine tail is added to the chez coding region. The pTet promoter can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2701
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 886
    Illegal AgeI site found at 2395
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/composite/uncategorized
//function/reporter
Parameters
n/ainvasin (1-625aa)_RFP-terminators