Part:BBa_K4579001

P_Tac promoter + RBS

This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTac promoter upstream of a hammerhead ribozyme, the latter of which helps insulate the transcript from sequence-specific interference effects. The pTac promoter includes the lac operator that may be bound by a LacI repressor protein, causing repression of transcription. LacI is removed from the operator sites when bound by the inducer molecule IPTG, allowing for the selective induction of transcription in cells containing both pTac and the lacI gene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]