Coding

Part:BBa_K4586014

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 12:09, 21 September 2023 by Mahmoud sayed (Talk | contribs) (→‎Part Description)


CD63

Part Description

This part is the cell surface protein, which is a member of the tetraspanin family. It has numerous functions, such as being a marker for multivesicular bodies, quantifying extracellular vesicles, and can also be fused with other proteins to combine functional devices in the exosomal membrane.

Usage

We implemented this part in our design to form our loading system for the therapeutic agent to be loaded into exosomes. This loading system is composed of three elements: First, CD63, which is a transmembrane protein naturally present and highly expressed on the external surface of the exosome membrane, second L7Ae, which is a RNA-binding protein conjugated to the internal domain of the CD63 protein, in order to bind to the third element of the loading system present in the 3` end of our cargo, which is the C\D box or kink turn.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None