Coding

Part:BBa_K4586019

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 10:11, 21 September 2023 by Mohamed-Ab0elghare (Talk | contribs) (→‎Part description)


G4S linker

Part Description

This part codes for the poly-Glycine-Serine (G4S) linker peptide that is a type of flexible,unstructured synthetic peptide linker sequence. G4S linker is composed of a core pentapeptide Gly-Gly-Gly-Gly-Ser that is repeated and usually found within scFV-based synthetic receptors as CARs and syn notch receptors.

Usage

We used this basic part in our design to mediate the transfer of our transcription module VP64 into the nucleus to carry out its action on the target promoter that regulates the expression of our therapeutic agent (Cass12k).




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None