Coding

Part:BBa_K4586002

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 09:51, 21 September 2023 by Mohamed-Ab0elghare (Talk | contribs) (→‎Part Description)


ZF21.16


Part Description

Zinc finger proteins are considered one of the most unique and huge collections of proteins that are variable in their biological function and structure. In our condition this zinc finger protein recognizes a specific DNA sequence thus ZF21.16 can be combined with any other protein as transcription factor to direct its activity toward the target sequences that are located near the gene of interest.

Usage

This part is implemented in our design to represent the internal domain of the syn notch receptor, which controls the level of our therapeutic agent by releasing the transcription factor VP64, which triggers the expression of the internal circuit that secretes the exosome's cargo, which is regulated by ZF 21-16 promoter activity, which is composed of three main components.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None