Composite

Part:BBa_K4806224

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-20)
Revision as of 09:11, 21 September 2023 by Lulang (Talk | contribs)


CYP9Q3 gene with mNeonGreen for Chlamydomonas reinhardtii (Phytobrick)


This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of CYP9Q3 (BBa_K4806004), mNeonGreen (BBa_K4806006) and the tRPL23-terminator (BBa_K3002006)*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).


Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
    Illegal PstI site found at 3519
    Illegal PstI site found at 3926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
    Illegal PstI site found at 3519
    Illegal PstI site found at 3926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2533
    Illegal BamHI site found at 3535
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
    Illegal PstI site found at 3519
    Illegal PstI site found at 3926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
    Illegal PstI site found at 3519
    Illegal PstI site found at 3926
    Illegal NgoMIV site found at 2948
    Illegal NgoMIV site found at 4719
    Illegal AgeI site found at 268
    Illegal AgeI site found at 3864
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

[edit]
Categories
Parameters
None