Part:BBa_K4806223

CYP9Q3 gene with mStop for Chlamydomonas reinhardtii (Phytobrick)

This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of CYP9Q3 (BBa_K4806004), mStop (BBa_K4806009) and the tRPL23-terminator (BBa_K3002006)^*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).

Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2533
  Illegal BamHI site found at 3222
  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  Illegal NgoMIV site found at 2948
  Illegal NgoMIV site found at 3555
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]