Coding

Part:BBa_K4586012

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 09:01, 21 September 2023 by Mahmoud sayed (Talk | contribs) (→‎Usage)


NadB

Part Description

NadB is the first enzyme in the NAD biosynthesis pathway that is useful in the production of ATP by increasing the metabolism rate of gene-modified cells through the FAD-dependent oxidation of L-aspartate to iminoaspartate, which has an essential role in exosome synthesis.

Usage

This part is implemented in our system to improve the efficacy of our therapeutic agent by increasing the default level of exosome synthesis within our engineered MSCs. This enzyme can therefore speed up the metabolism of gene-modified cells and, in our project, also accelerate exosome biogenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 406
    Illegal AgeI site found at 1026
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None