Coding

Part:BBa_K4891015

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-07-03)
Revision as of 01:47, 20 September 2023 by KexinFei (Talk | contribs)


ptsGup-glk-glf-ptsGdown

It is the key part that is responsible for strengthening glucose utilization rate. The phosphotransferase system was replaced by heterogeneously introducing glucose facilitator in strain E. coli ptsG::glk-glf.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1818
    Illegal AgeI site found at 1974
    Illegal AgeI site found at 1986
    Illegal AgeI site found at 2697
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None