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Part:BBa_K4891012:Design

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)
Revision as of 01:22, 20 September 2023 by KexinFei (Talk | contribs)

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poxB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 651
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 651
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.


Source

ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.

References