Coding
Part:BBa_K4606005
Designed by: Litong, Wu Group: iGEM23_Nanjing-NFLS (2023-09-14)
miR-3074-5p coding sequence
Description
Here we construct the sequences that encoding miR-3074-5p. The gene sequence encoding miRNA is first transcribed into pri-miRNA, which is cleaved and modified by ribonuclease to become the final miRNA. MiRNAs are a class of endogenous non-coding single-stranded small RNAs with a length of approximately 20–22 nucleotides and their expression changes are related to many human diseases.
After inserting this sequence into the vector and expressing it in cells, we could test whether the miR-3074-5p was able to target the SMAD4 gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |