Part:BBa_K4719009
pKARA_RT3 styrene monooxigenase
Introduction
Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in Komagataeibacter xylinus for in vivo bacterial cellulose polymer composition modification. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry.
Colorful cellulose was made by introducing styrene monooxygenase pKARA_RT3 BBa_K4719018 to K. xylinus. This enzyme can metabolize indigo and its other derivatives into indigo dyes. Bacteria produce cellulose alongside pigments. Since they are not water soluble, the final product retains the color.
Usage and Biology
pKARA_RT3 is styrene monooxygenase. Styrene monooxygenases belong to oxidoreductases, specifically to an enzyme class 1.14.14-, which act on paired donors, with incorporation or reduction of molecular oxygen, which is donated by reduced flavin or flavoprotein. Styrene monooxygenases have been shown to produce indigo - a blue dye (1).
References
1. O’Connor, K.E., Dobson, A.D. and Hartmans, S. (1997) ‘Indigo formation by microorganisms expressing styrene monooxygenase activity’, Applied and Environmental Microbiology, 63(11), pp. 4287–4291. doi:10.1128/aem.63.11.4287-4291.1997.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1171
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1171
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1171
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1171
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1171
Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 448
Illegal AgeI site found at 634 - 1000COMPATIBLE WITH RFC[1000]
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