Plasmid

Part:BBa_K4605004

Designed by: Jinran Liu   Group: iGEM23_Nanjing-China   (2023-09-06)
Revision as of 09:24, 17 September 2023 by ManovaH0 (Talk | contribs)


Producing indigo in K.xylinus

Description

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 78
    Illegal EcoRI site found at 3809
    Illegal SpeI site found at 3933
    Illegal PstI site found at 3947
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 78
    Illegal EcoRI site found at 3809
    Illegal NheI site found at 34
    Illegal SpeI site found at 3933
    Illegal PstI site found at 3947
    Illegal NotI site found at 3940
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 78
    Illegal EcoRI site found at 3809
    Illegal XhoI site found at 3105
    Illegal XhoI site found at 3804
    Illegal XhoI site found at 4964
    Illegal XhoI site found at 5990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3933
    Illegal EcoRI site found at 78
    Illegal EcoRI site found at 3809
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 78
    Illegal EcoRI site found at 3809
    Illegal SpeI site found at 3933
    Illegal PstI site found at 3947
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 758
    Illegal AgeI site found at 2016
    Illegal AgeI site found at 2069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI site found at 1605
    Illegal BsaI site found at 2319
    Illegal BsaI.rc site found at 423
    Illegal BsaI.rc site found at 3609
    Illegal BsaI.rc site found at 6136
    Illegal SapI.rc site found at 1857


[edit]
Categories
Parameters
None