RNA

Part:BBa_K4789000:Design

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-17)
Revision as of 08:02, 17 September 2023 by Sunny Duan (Talk | contribs) (→‎References)


miR-22-sponge


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Refer to Yang, et.al 2019.


Source

The plasmid of pCDNA3-MCS-nPepper was obtained from Naying Biotechnology Company.

References

Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293.