Composite
Part:BBa_K4743005:Design
Designed by: Jen Hsien, Liu Group: iGEM23_PTSH-Taiwan (2023-09-16)
NadV+ADH1 terminator with adapter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1689
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1689
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1689
Illegal BglII site found at 515
Illegal BamHI site found at 16
Illegal BamHI site found at 367
Illegal BamHI site found at 975 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1689
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1689
Illegal AgeI site found at 1702 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We choose the NAMPT from Ralstonia solanacearum, because it has the highest activity compare to other organism's NAMPT.
Source
Ralstonia solanacearum