Composite
Part:BBa_K4743003:Design
Designed by: Jen Hsien, Liu Group: iGEM23_PTSH-Taiwan (2023-09-16)
NadB+ADH1 terminator with adapter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1833
Illegal XbaI site found at 702
Illegal XbaI site found at 921
Illegal XbaI site found at 1167
Illegal XbaI site found at 1264
Illegal SpeI site found at 1638
Illegal PstI site found at 1034 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1833
Illegal SpeI site found at 1638
Illegal PstI site found at 1034 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1833
Illegal BamHI site found at 16 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1833
Illegal XbaI site found at 702
Illegal XbaI site found at 921
Illegal XbaI site found at 1167
Illegal XbaI site found at 1264
Illegal SpeI site found at 1638
Illegal PstI site found at 1034 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1833
Illegal XbaI site found at 702
Illegal XbaI site found at 921
Illegal XbaI site found at 1167
Illegal XbaI site found at 1264
Illegal SpeI site found at 1638
Illegal PstI site found at 1034
Illegal AgeI site found at 1846 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 801
Design Notes
No specific consider for the design
Source
Listeria innocua (serotype 6a)