Protein_Domain
DigA
Part:BBa_K243003:Design
Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-12)
Digoxigenin binding protein (DigA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Freiburg standard25 part
Source
synthesized by purimex
LOCUS His+FluA+Split+F 3616 bp DNA circular SYN 08-OCT-2009 DEFINITION Ligation of His+FluA+Split+FokI into pMA2_NgoMIV_SpeI ACCESSION His+FluA+Split+F KEYWORDS . SOURCE Unknown. ORGANISM Unknown
Unclassified.
REFERENCE 1 (bases 1 to 3616)
AUTHORS Self JOURNAL Unpublished.
COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI
Fragment ends: SpeI and NgoMIV
Fragment size: 2412
Insert molecule: His+FluA+Split+FokI
Fragment ends: NgoMIV and SpeI
Fragment size: 1204
FEATURES Location/Qualifiers
CDS 129..989
/gene="AmpR"
/SECDrawAs="Gene"
CDS 2008..3213
/gene="ORF-1"
/SECDrawAs="Gene"
CDS 2017..2034
/gene="His Tag"
/SECDrawAs="Gene"
CDS 2041..2562
/gene="FluA"
/SECDrawAs="Gene"
CDS 2569..2619
/gene="Split-Linker"
/SECDrawAs="Gene"
CDS 2623..3212
/gene="'Fok inaktiv'"
/SECDrawAs="Gene"
ORIGIN
1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc
2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc
2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc
2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac
2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag
2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac
2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac
2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag
2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg
2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggccg accagcctgt
2581 aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag
2641 gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg
2701 atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc
2761 ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa accagcaggt
2821 gccatttata ccgttggttc cccgatcgat tatggcgtta tcgtggccac aaaagcgtat
2881 tctggcggtt ataatctgcc gattggtcag gctgatgaga tggaacgtta tgtggaagag
2941 aatcagaccc gtaacaaaca tctgaacccg aacgaatggt ggaaagtgta tccgtcaagt
3001 gtcaccgagt tcaaatttct gttcgtgagc ggccacttta aaggcaacta taaagcccag
3061 ctgactcgtc tgaaccatat caccaatagc aatggggcag tgctgagtgt tgaggaactg
3121 ctgatcggtg gagaaatgat caaagcaggc accctgactc tggaagaagt tcgccgtaaa
3181 ttcaacaatg gcgagatcaa ttttaccggt taatactagt agcggccgct gcagagctca
3241 tggcgcgcct aggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt
3301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
3361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
3421 ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg
3481 gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
3541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
3601 aacgcttaca atttag
//