Plasmid

Part:BBa_K4606000:Design

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-08-04)
Revision as of 12:44, 14 September 2023 by JerryLi (Talk | contribs) (→‎Design Notes)

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Vector-AmpR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1163
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1163
    Illegal NgoMIV site found at 487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 279
    Illegal BsaI.rc site found at 2637
    Illegal SapI site found at 1557


Design Notes

Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin. To use this device, all you need to do is cut the plasmid at XbaI and insert your target fragment into this position. We have proved its feasibility by inserting our destination fragment (Firefly Luciferase) to the plasmid. The recombinants show resistance to Ampicillin.

Source

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References