Part:BBa_K4806001

CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (BBa_K4806013) and a terminator like tRPL23 (BBa_K3002006), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (BBa_K4806012) is recommended.

Constructs

Fig.1 Construct design
We designed 5 level 2 parts containing CYP2D6 using the modular cloning system (MoClo).

Here are the links to the built constructs: and transformed these into Chlamydomonas reinhardtii.

• 1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) ()
• 2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) ()
• 3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) ()
• 4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) ()
• 5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) ()