Coding

Part:BBa_K4891001:Design

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)
Revision as of 09:23, 13 September 2023 by KexinFei (Talk | contribs) (→‎Source)


aroB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 906
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.


Source

This gene is originated from Escherichia coli MG1655.

References