Coding
Part:BBa_K4891000:Design
Designed by: Ruyi Shi Group: iGEM23_Guangzhou-MedX (2023-06-13)
aroGfbr
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 934
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 241
Design Notes
There are three DAHP synthase in E. coli MG1655, and these enzymes are feedback-inhibited by L-phenylalanine, L-tyrosine and L-tryptophan. Therefore, AroGfbr usage could alleviate this effect.
Source
This gene is originated from Escherichia coli MG1655.