Coding

Part:BBa_K4806001:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-13)
Revision as of 07:53, 13 September 2023 by Lulang (Talk | contribs)


CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NotI site found at 862
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 1348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/)
We inserted a few mutations to eliminate the recognition sites for our type IIS restriction enzymes BbsI and BsaI.


Source

https://www.uniprot.org/uniprotkb/P10635/entry

References