Part:BBa_K4656003

Lrp

The Lrp is a gene encoding leucine reactive protein, which is expressed by E. coli itself. It mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons, and Lrp mediates this effect for at least some of these operons. For example, it is a regulator of the branched-chain amino acid transport genes. In our project, Lrp dimerizes with butyrate, binds to pPchA promoter, and further produces pchA.

Usage and Biology

LRP, as a component of the natural FIM system of E. coli, is a fimS regulator. Both LRP and IHF (Integrated Host factor) may act to regulate the actual inversion events through an involved DNA binding protein complex. In addition, there is the OFF-to-ON inhibitory protein papB and many other hypothesized and known regulators that directly act on LRP. During the course of the project, the FIM system can be considered. (Additional background on FIM systems can be found in the references [1])

Reference

[1]. Schwan W. R. (2011). Regulation of fim genes in uropathogenic Escherichia coli. World journal of clinical infectious diseases, 1(1), 17–25. https://doi.org/10.5495/wjcid.v1.i1.17

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Unknown
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 30
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]