Part:BBa_K4719005
GNA1
Introduction
Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in Komagataeibacter xylinus for in vivo bacterial cellulose polymer composition modification. Firstly, we chose to produce a cellulose-chitin polymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design.
Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine, which can be recognized as a viable substrate for cellulose synthase and incorporated in bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium BBa_K4719013, and the second one was the production of N-acetylglucosamine by K. xylinus from simple sugars such as glucose, fructose, and saccharose in the growth medium BBa_K4719014.
Usage and Biology
GNA1 is glucosamine 6-phosphate N-acetyltransferase. This enzyme catalyzes the transfer of an acetyl group from acetyl coenzyme A to glucosamine-6-phosphate to form N-acetylglucosamine-6-phosphate, which is an essential intermediate in UDP-GlcNAc biosynthesis. GNA1 is a part in BBa_K4719014.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 118
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 118
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 118
Illegal BamHI site found at 457 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 118
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 118
- 1000COMPATIBLE WITH RFC[1000]
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