Composite

Part:BBa_K4719020

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-07)
Revision as of 08:06, 7 September 2023 by Augustestankeviciute (Talk | contribs)


CBD-ProThr box-ArCE4A chitin deacetylase and cellulose binding domain fusion protein

We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 432
    Illegal NgoMIV site found at 961
    Illegal AgeI site found at 417
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None