Part:BBa_K4607008

PCNP

Description

The biobrick consists of the polycationic nonapeptide (PCNP) which is capable to destabilize the lipopolysaccharide binds on Gram-negative baterium. The principle about the PCNP is related to the ionic interactions between the phosphate groups, divalent cations, and hyrdophobic lipids' stacking, where the PCNP acts as a destabilizing. This part has been fused with endolysins in order to increase their capability to lyse Gram-negative bacterium, demonstrating that the addition of the PCNP allows the endolysin introduction into the bacteria cell membrane. It part have been evaluated in Escherichia coli. The peptide has a lenght of nine amino acids [1].

imagen Figure 1. PCNP diagram.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

References

[1] Briers, Y., Walmagh, M., Van Puyenbroeck, V., Cornelissen, A., Cenens, W., Aertsen, A., Oliveira, H., Azeredo, J., Verween, G., Pirnay, J.-P., Miller, S., Volckaert, G., & Lavigne, R. (2014). Engineered endolysin-based “Artilysins” to combat multidrug-resistant gram-negative pathogens. MBio, 5(4), e01379-01314. https://doi.org/10.1128/mBio.01379-14 ‌

===References===