Coding

Part:BBa_K4607008

Designed by: Axel Rojero   Group: iGEM23_Tec-Chihuahua   (2023-09-04)
Revision as of 19:26, 6 September 2023 by Axelrohz24 (Talk | contribs) (→‎Usage and Biology)


PCNP

Description


The biobrick consists of the polycationic nonapeptide (PCNP) which is capable to destabilize the lipopolysaccharide binds on Gram-negative baterium. The principle about the PCNP is related to the ionic interactions between the phosphate groups, divalent cations, and hyrdophobic lipids' stacking, where the PCNP acts as a destabilizing. This part has been fused with endolysins in order to increase their capability to lyse Gram-negative bacterium, demonstrating that the addition of the PCNP allows the endolysin introduction into the bacteria cell membrane. It part have been evaluated in Escherichia coli. The peptide has a lenght of nine amino acids.


imagen
Figure 1. PCNP diagram.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

===References===
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Categories
Parameters
None