Composite

Part:BBa_K4719017

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-03)
Revision as of 17:35, 3 September 2023 by Registry (Talk | contribs)

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phaCAB operon for polyhydroxybutyrate synthesis in K. xylinus

Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331b backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
    Illegal NotI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 642
    Illegal BamHI site found at 3039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 368
    Illegal NgoMIV site found at 602
    Illegal NgoMIV site found at 914
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1606
    Illegal NgoMIV site found at 1673
    Illegal AgeI site found at 341
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None