![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4719017
Designed by: Auguste Stankeviciute Group: iGEM23_Vilnius-Lithuania (2023-09-03)
phaCAB operon for polyhydroxybutyrate synthesis in K. xylinus
Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331b backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
Illegal NotI site found at 200 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 642
Illegal BamHI site found at 3039 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 368
Illegal NgoMIV site found at 602
Illegal NgoMIV site found at 914
Illegal NgoMIV site found at 1193
Illegal NgoMIV site found at 1606
Illegal NgoMIV site found at 1673
Illegal AgeI site found at 341 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |