Composite

Part:BBa_K4719014

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-03)
Revision as of 13:26, 3 September 2023 by Registry (Talk | contribs)

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AGM1-GFA1-GNA1-UAP1 operon for synthesis of bacterial cellulose-chitin copolymer

This operon allows K. xylinus to synthesize an excess of UDP-N-Acetylglucosamine from simple sugars in the growth medium. UDP-N-Acetylglucosamine can be used by cellulose synthase to produce bacterial cellulose-chitin polymer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal BglII site found at 1484
    Illegal BglII site found at 1583
    Illegal BglII site found at 3033
    Illegal BamHI site found at 4339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None