![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4719014
Designed by: Auguste Stankeviciute Group: iGEM23_Vilnius-Lithuania (2023-09-03)
AGM1-GFA1-GNA1-UAP1 operon for synthesis of bacterial cellulose-chitin copolymer
This operon allows K. xylinus to synthesize an excess of UDP-N-Acetylglucosamine from simple sugars in the growth medium. UDP-N-Acetylglucosamine can be used by cellulose synthase to produce bacterial cellulose-chitin polymer.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2076
Illegal EcoRI site found at 2574
Illegal EcoRI site found at 4000
Illegal EcoRI site found at 4929
Illegal EcoRI site found at 5091
Illegal XbaI site found at 3174
Illegal SpeI site found at 37
Illegal SpeI site found at 4867
Illegal SpeI site found at 5755 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2076
Illegal EcoRI site found at 2574
Illegal EcoRI site found at 4000
Illegal EcoRI site found at 4929
Illegal EcoRI site found at 5091
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal SpeI site found at 4867
Illegal SpeI site found at 5755 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2076
Illegal EcoRI site found at 2574
Illegal EcoRI site found at 4000
Illegal EcoRI site found at 4929
Illegal EcoRI site found at 5091
Illegal BglII site found at 1484
Illegal BglII site found at 1583
Illegal BglII site found at 3033
Illegal BamHI site found at 4339 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2076
Illegal EcoRI site found at 2574
Illegal EcoRI site found at 4000
Illegal EcoRI site found at 4929
Illegal EcoRI site found at 5091
Illegal XbaI site found at 3174
Illegal SpeI site found at 37
Illegal SpeI site found at 4867
Illegal SpeI site found at 5755 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2076
Illegal EcoRI site found at 2574
Illegal EcoRI site found at 4000
Illegal EcoRI site found at 4929
Illegal EcoRI site found at 5091
Illegal XbaI site found at 3174
Illegal SpeI site found at 37
Illegal SpeI site found at 4867
Illegal SpeI site found at 5755 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |