Coding

Part:BBa_K4804000

Designed by: Jing Shi   Group: iGEM23_Bioplus-China   (2023-08-22)
Revision as of 05:37, 1 September 2023 by Jing2023 (Talk | contribs)


PETase-R

PETase has PET (poly(ethylene terephtalate) degradation activity first identified in Ideonella sakaiensis. R is the DNA sequence of a repetitive region of PySp1 from the piriform glandular filament of Arachnid. The function of the repeat area is mainly related to the mechanical properties of spider silk fiber.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 871
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 923
    Illegal NgoMIV site found at 965
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None