Composite

Part:BBa_K180004

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-16)
Revision as of 10:24, 16 October 2009 by Rich (Talk | contribs)

Game of Life - Primary plasmid

Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.


Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 945
    Illegal NheI site found at 968
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 2891


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controllux
directionforward
emission511 nm
emit511
excitation501 nm
excite501
lum
proteinGFPmut3b
tagLVA