Composite
Part:BBa_K180004
Designed by: Richard Wilson, Stephanie Chambers Group: iGEM09_Southampton (2009-10-16)
Game of Life - Primary plasmid
Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.
Usage and Biology
This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 945
Illegal NheI site found at 968 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2215
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Illegal BsaI.rc site found at 2891
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/bidirectional
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/bidirectional
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
control | lux |
direction | forward |
emission | 511 nm |
emit | 511 |
excitation | 501 nm |
excite | 501 |
lum | |
protein | GFPmut3b |
tag | LVA |