Coding
Part:BBa_K4880003:Design
Designed by: Xinran Xia Group: iGEM23_BASIS-China (2023-08-25)
PtPS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1161
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1828
Design Notes
We assembled it with Ptrc and Theophylline-dependent riboswitch.
Source
This sequence is derived from the Pinus taeda genome, and it is adapted to the cyanobacteria codon. We took advantage of the publication of Blanc-Garin et al. (2022).