Coding

Part:BBa_K4880002:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-08-25)
Revision as of 03:20, 26 August 2023 by Registry (Talk | contribs)

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AgBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1681
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1681
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 64
    Illegal BsaI.rc site found at 505
    Illegal BsaI.rc site found at 1288


Design Notes

We assembled it with Ptrc and Theophylline-dependent riboswitch.


Source

This sequence is derived from the Abies grandis genome, and it is adapted to the cyanobacteria codon. We took advantage of the publication of Blanc-Garin et al. (2022).


References