Part:BBa_K4727000

A. baumannii & K. pneumoniae standard backbone

This plasmid backbone was developed by Wang et al [1], it is provided of two ORIs: the first one a pMB1/pUC high copy number ORI for E. coli, the second one is capable o maintaining the plasmid both in Acinetobacter baumannii and Klebsiella pneumoniae

This backbone has been made standard RFC[10] compatible and provided with the RFC[10] prefix and suffix. Flanked by prefix and suffix is located part BBa_J23119 that has been demonstrated able to promote mRNA synthesis in K. pneumoniae and A. baumannii

The plasmid brings AmpR as a selection marker

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Example Image

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Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 3664
  Illegal suffix found at 3721
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3664
  Illegal NheI site found at 969
  Illegal NheI site found at 3692
  Illegal NheI site found at 3715
  Illegal SpeI site found at 3722
  Illegal PstI site found at 3736
  Illegal NotI site found at 3670
  Illegal NotI site found at 3729
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3664
  Illegal BglII site found at 1162
  Illegal XhoI site found at 1775
  Illegal XhoI site found at 3780
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3664
  Illegal suffix found at 3722
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3664
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3679
  Illegal SpeI site found at 3722
  Illegal PstI site found at 3736
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI site found at 4008