Coding

Part:BBa_K4593001:Design

Designed by: Jianfei Song   Group: iGEM23_BNDS-China   (2023-08-21)
Revision as of 20:22, 21 August 2023 by Registry (Talk | contribs)

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Spn1s_LysRZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 890
    Illegal AgeI site found at 1028
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Biobrick assembly, Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

Genome of bacteriophage SPN1S.

References