Primer

Part:BBa_K4790016

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-08-21)
Revision as of 14:22, 21 August 2023 by Registry (Talk | contribs)

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ASR1-L246H-R

The primer was designed to perform site-directed mutation which was defined as a reverse primer. It can mutate base sequence which is on code 59 and coding the 246th amino acid from leucine to histidine. According to Codon preference in E.coli, ATG has the highest possibility coding histidine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None