Primer
Part:BBa_K4790016
Designed by: Hongyan Jing Group: iGEM23_NNU-CHINA (2023-08-21)
ASR1-L246H-R
The primer was designed to perform site-directed mutation which was defined as a reverse primer. It can mutate base sequence which is on code 59 and coding the 246th amino acid from leucine to histidine. According to Codon preference in E.coli, ATG has the highest possibility coding histidine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |