Composite

Part:BBa_K4905009

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-08-14)
Revision as of 09:07, 16 August 2023 by VeerleJegers (Talk | contribs)


Elastin-Like Polypeptide Triblock with Leucine Zippers

This part is made up of parts BBa_K4905005, BBa_K4905001 and BBa_K4905002. It was used by the TU Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.

The construct consists of Elastin-like Polypeptides (ELPs) and two leucine zippers that have affinity for each other. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a reversible hydrogel.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 99
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1982
    Illegal XhoI site found at 1999
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 99
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 99
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 336
    Illegal NgoMIV site found at 426
    Illegal NgoMIV site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None