Composite

Part:BBa_K4905015

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-08-15)
Revision as of 11:14, 15 August 2023 by Registry (Talk | contribs)

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Elastin-Like Polypeptide Triblock with FRB

This part is made up of parts BBa_K4905001 BBa_K4905002 and BBa_J18926. It was used by the Eindhoven 2023 team to form a hydrogel outside as well as inside e.coli BL21 cells.

The construct consists of Elastin-like Polypeptides (ELPs) and rapamyacin binding domains. Rapamyacin is a known molecular glue that can be used to link FRB and FKBP12 protein domains together. ELPs have hydrophilic and hydrophobic domains that exhibit reversible phase separation behavior that is temperature dependent. This allows them to be used in the formation of a hydrogel.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2177
    Illegal XbaI site found at 294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2177
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2177
    Illegal BglII site found at 225
    Illegal BglII site found at 4243
    Illegal XhoI site found at 2194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2177
    Illegal XbaI site found at 294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2177
    Illegal XbaI site found at 294
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 531
    Illegal NgoMIV site found at 621
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 3123
    Illegal NgoMIV site found at 3300
    Illegal NgoMIV site found at 3390
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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