Coding

Part:BBa_K4652008:Design

Designed by: YUAN-AN CHEN   Group: iGEM23_Mingdao   (2023-08-15)
Revision as of 06:05, 15 August 2023 by Chen-yuanan (Talk | contribs)

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SpyTag-PCLase1-SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Synthesized by Integrated DNA Technologies, Inc. The DNA sequence for PCLase1 is from a Lipase in Pseudomonas hydrolytica with deletions of start codon (ATG) and stop codon (TAA). SpyTag and SpyCatcher are from CnaB domains of fibronectin-binding (FbaB) protein in Streptococcus pyogenes.

References