Coding

Part:BBa_K4790060

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)
Revision as of 08:39, 14 August 2023 by Registry (Talk | contribs)

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ASR1(node 59)-N81H

The DNA fragment has been site-directed mutated through PCR. The 81st amino acid in expression product of node 59 was mutated from aspartic acid to histidine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None