Primer

Part:BBa_K4790048

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)
Revision as of 07:41, 14 August 2023 by ZWing (Talk | contribs)


ASR1-N81H-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding histidine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None