Coding

Part:BBa_K4765902

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-11)
Revision as of 08:09, 11 August 2023 by Siliang Zhan (Talk | contribs)


MysA codon optimized

Usage and Biology

Similar to BBa_K4765012(initimin-Nb2 fusion), we replaced Nb2 with Nb1 in order to interact with BBa_K4765009(initimin+Ag1 fusion) to create a biofilm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1198


[edit]
Categories
Parameters
None