Coding

Part:BBa_K4765014

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)
Revision as of 07:54, 11 August 2023 by Siliang Zhan (Talk | contribs)


INPNC

Introduction

Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs[1].

Usage and Biology

We performed codon optimization on BBa_K4273014 (NlmysH) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 481
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.
[edit]
Categories
//cds/enzyme
Parameters
protein