Part:BBa_K4765011

MysB codon optimized

Introduction

MysB (O-MT), an O-methyltrans-ferase, converts Demethyl 4-deoxygadusol into 4-deoxygadusol (4-DG), completing the production of 4-DG, the basic substrate of MAAs^[1].

Usage and Biology

We performed codon optimization on BBa_K4273005 (NpR5599) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysB catalyzes the second reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 532

References

↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.

[1] Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.

[1]